![]() FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). Typically flow cytometers generate output files in FCS format ( e.g. Is special software required for data analysis? LEGENDplex™ kits are guaranteed for 6 months from the date of receipt, but may have a shelf life of up to 2 years from the date of manufacture. ![]() ![]() What is the shelf life of LEGENDplex™ kits? Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal. There may be a decrease in signal, but overall, the assay results should not be affected. The samples can be kept overnight at 4☌ while being protected from exposure to light and be read the next day. Polystyrene ELISA or cell culture plates should not be used.Īfter I finish the staining process, how long can I wait before reading my LEGENDplex™ samples? All plates should be made from low binding polypropylene. However, if you don’t have access to a vacuum manifold or if you prefer, then you can use the V-bottom plates and follow the recommended assay protocols for the type of plates you choose. A vacuum filtration unit is required to work with the filter plates. Should I perform the assay with the filter plates or with V-bottom plates?įilter plates or V-bottom plates have been included in some kits for your convenience. Alternatively, you can remove the liquid by using a pipette. After centrifugation using a swinging-bucket rotor with a plate adaptor, you can remove the liquid by flicking the plate quickly, dumping the contents into a sink, and patting it dry carefully on a stack of clean paper towels without losing the beads. If you do not have a vacuum, the assay should be run in a V-bottom plate. If I don't have a vacuum, how do I remove the liquid from my plate?
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